2012-2013生物化学A卷(16K) 2

山东农业大学课程考试专用 2012 -2013 学年第 一 学期 《生物化学A》试题（A卷） 课程代码 BB035312 考试方式 闭卷 考试时长 100 分钟 姓名 学号 教学班号 专业 级 班 题 号 满 分 得 分 阅卷人 一 15 二 25 三 30 四 30 合计 100 一、 Write Out the Chinese Name of the Following Biochemical Terms (写出下列名词或英文简写符号的中文名称) (每空1分，共15分) 中文名称 英文名称 Isoelectric Point cytochrome oxidase ACP energy currency APS Nucleic Acid Hybridization reduction potential lagging strand hnRNA Synonym codon pyruvate kinase isocitrate dehydrogenase feed-back inhibition The glyoxylate cycle Carnitine shuttle 二、Single Choice Questions(单项选择题)（每小题1分，共25分） 注：考试期间试卷不允许拆开。 第 1 页 共 7 页 山东农业大学课程考试专用 1 16 2 17 3 18 4 19 5 20 6 21 7 22 8 23 9 24 10 25 11 12 13 14 15 1. This functional group of is ( ). A. ketone B. aldehyde C. carboxylic acid D. ester 2. Mitochondria and chloroplasts share a number of features in common. Which of the following is NOT one of them? ( ) A. Both are found in all eukaryotic cells. B. Both are surrounded by a double membrane. C. Both contain DNA. D. Both may have been derived from symbiotic bacteria that invaded primitive eukaryotic cells. 3. Which of the following amino acids strongly absorbs UV light? ( ) A. Phe B. Tyr C. Trp D. all of the above 4. The isoelectric point of a protein is defined as ( ) A. the pH at which the net charge on the molecule is 0 B. the pH at which all groups are protonated C. the pH at which all groups are unprotonated D. the pH at which each acidic group is protonated and each basic group is unprotonated 5. What kind of amino acids is likely to be found on the surface of a globular protein? A. aliphatic amino acids B. Aromatic amino acids C. Charged amino acids D. None of the above 6. Free rotation about the peptide bond in a protein is restricted primarily because of ( ) A. partial double-bond character of the peptide bond. B. hydrogen bonding to the amide backbone groups. C. partial double-bond character of the N-C bond. D. restrictions caused by local folding patterns. 7. Which of the following amino acids is least likely to be found in an extended alpha-helix? ( ) A. proline B. Valine C. arginine D. serine 8. Which of the following statements about multisubunit globular proteins is incorrect? ( ) 注：考试期间试卷不允许拆开。 第 2 页 共 7 页 山东农业大学课程考试专用 A. Multisubunit enzymes often assemble the active site with residues from adjacent subunits. B. Oligomers are more stable than subunit monomers. C. Errors during protein synthesis are minimized by the synthesis of shorter proteins. D. Subunits are typically held together by covalent interactions. 9. The identification of a protein sequence ( ) A. requires some preliminary knowledge of its tertiary structure B. proceeds in a carboxyl to amino end direction C. Chemically labeled amino acids at the amino end of a protein are released by acid hydrolysis and then identified by chromatography D. is based on the same principle as DNA sequencing by chain termination 10. Gel electrophoresis is a method that ( ) A. separates proteins based on their differential velocity in an electrical field B. separates proteins by centrifuging a sample at high speed C. separates proteins independent of their size D. cannot be used to separate nucleic acids 11. Enzymes accelerate reaction rates by ( ) A. altering the equilibrium of a reaction. B. increasing the temperature of a solution. C. increasing entropy. D. decreasing the energy of the transition state. 12. Yeast can grow anaerobically(厌氧地) and produce ethanol from glucose. What pathway do they likely use for this conversion? A. glycolysis B. citric acid cycle C. oxidative phosphorylation D. gluconeogenesis 13. Which of the following enzymes catalyzes a substrate-level phosphorylation reaction? A. hexokinase B. pyruvate kinase C. glyceraldehyde-3-phosphate dehydrogenase D. phosphoglycerate kinase 14. Which of the following is true about the fatty acids? ( ) A. can be made from citric-acid cycle intermediates. B. can be used to replenish (补充) citric-acid cycle intermediates. C. can both be made from and replenish citric-acid cycle intermediates. D. Neither is made from nor replenish citric-acid cycle intermediates 15. Double-stranded nucleic acid helices （ ） A. do not have a handedness. B. can be either right- or left-handed. C. are always left-handed. D. are always right-handed. 16. Which is the major force to maintain the tertiary structure of a protein? ( ) 注：考试期间试卷不允许拆开。 第 3 页 共 7 页 山东农业大学课程考试专用 A. van der waals B. hydrophobic interaction C. hydrogen bond D. Ionic bonds 17. A distinctive feature of allosteric enzymes is ( ) A. lack of response to inhibitors B. cooperative behavior C. that they respond with conformational changes when ligands bind at sites away from the active site D. that they consist of one subunit only 18. When v0 = 1/4 Vmax ，Km = ( ) A. [S] B. 2[S] C. 3[S] D. 1/2[S] 19. The cofactor of transaminases is ( ) A. Tetrahydrofolate (H4 folate) B. S-Adenosylmethionine C. pyridoxal phosphate (PLP) D. NAD+ 20. The active form of glucose used in starch synthesis is ( ) A. G-6-P B. GDP-Glucose C. G-1-P D. ADP-Glucose 21. Which of the following chemical does NOT produce during the pentose phosphate pathway? ( ) A. sedoheptulose 7-phosphate B. ribulose 5-phosphate C. galactose 6-phosphate D. erythrose 4-phosphate 22. Which of the following statements about Oxidative Phosphorylation is NOT true ( ) A. The chemiosmotic theory explains the coupling of electron flow and ATP synthesis B. e- flow and ATP synthesis are separate events, coupled via a transmembrane H+ gradient C. 2,4-dinitrophenol disrupts the tight coupling between electron transport and the ATP synthase D. Oligomycin inhibits the e- flow. 23. Which description about σ factor is correct? ( ) A. It is a subunit of RNA polymerase complex, and it determines the initiation of transcription in prokaryotic cells. B. It is a subunit of DNA polymerase complex, and it leads to the 5'→3' and 3'→5' exonuclease of DNA polymerase. C. It is a subunit of ribosome complex, and it can binds to the mRNA. D. It is a linker of 30S subunit and 50S subunit of ribosome and leads to 70S ribosome formation. 24. Which of the following component that do not code for proteins can RNA molecules contain? ( ) 注：考试期间试卷不允许拆开。 第 4 页 共 7 页