苯磺隆对斑马鱼胚胎发育的影响

3讨论

苯磺隆由于对麦田阔叶杂草的生长抑制效果十分良好，且用量低，对生物的毒性作用较低，近年来被国内外广泛使用。由于苯磺隆的降解速度较为缓慢，降解半衰期1-2个月，残留较高，随着雨水浇淋，土壤的渗透作用，势必会对环境造成污染，通过食物链危害生物的健康。然而，苯磺隆作为新型的高效除草剂，其毒理学研究还十分有限。

本实验利用斑马鱼胚胎对苯磺隆毒理学效应进行了初步研究。结果表明，不同浓度的苯磺隆溶液均可降低斑马鱼胚胎孵化率，而且其影响表现出明显的剂量依赖效应，即苯磺隆浓度越到，斑马鱼胚胎孵化率越低。实验还发现，三种处理组中胚胎开始孵化时间和孵化完毕时间均相同，而且低浓度苯磺隆对斑马鱼胚胎集中孵化时间也没有影响，这些结果再次证明了苯磺隆的毒性作用较低[2-4]。然而，随着浓度升高，胚胎集中孵化时间会推迟，表明高浓度苯磺隆可延缓斑马鱼胚胎发育进程。但关于其对胚胎发育过程中的组织损伤、畸形等还需要进一步研究，以深入认识烟嘧磺隆的毒理学效应。

(指导教师：王志平)

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Influence of Tribenuron-methyl on the Hatching of Zebrafish

Embryos

ZHAO Jin-long

(Class 1 Grade 2009, Department of Life Science, College of Chemistry and Life

science, Weinan Normal University)

Abstract: This experiment is on zebrafish, to research the effect of tribenuron-methyl on zebrafish embryos incubation. In the experiment, three different concentrations (80mg/L, 120mg/L, 160mg/L) of tribenuron-methyl solution is treated to the zebrafish embryos, studying the tribenuron-methyl solution's effect on the embryos embryos. Summarizing, we have found that the hatching rate of zebrafish embryos decreased with increasing the concentration of tribenuron-methyl solution, resulted in slowing down the process of embryonic development along with the postponement of the incubation time.

Key words: tribenuron-methyl; zebrafish embryos; hatching rate; individual development