药科大学教案(药物分析试验首页1)

1. 紫外分光光度仪的使用有哪些注意事项？ 2. 高效液相色谱仪的使用有哪些注意事项？ 教具及教学设备要求

100ml容量瓶，50ml容量瓶，电子天平，100ml量筒，10ml移液管，紫外分光光度仪，1cm 比色皿，高效液相色谱仪

Experiment 7 The Assay of propranolol hydrochloride tablet

I. Purpose

1. To review the UV method to determine the content of tablet. 2. To review the HPLC method to determine the content of tablet.

2. Contents and teach time assignment

1、Determination of propranolol hydrochloride by UV method

Propranolol Hydrochloride Tablets contain not less than 90.0percent and not more than 110.0percent of the labeled amount of C16H21NO2·HCl. Assay-------------------------------------------------------(3.5hour) Sample solution (1), (2) preparation

Measure the absorbance at the maximum at 290 nm, Appendix II B. Calculate the content of C16H21NO2,HCl

2、Determination of propranolol hydrochloride by HPLC method

Assay-------------------------------------------------------(3.5hour)

Carry out the method for high performance liquid chromatography (Appendix V D)

References

BP2003, USP24

Focal point

The use of UV spectrometer

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The use of high performance liquid chromatography

Discussion

1. What are the comments of the UV spectrometer?

2. What are the comments in the use of high performance liquid chromatography

teaching device

100ml volumetric flask 50ml volumetric flask electronic balance 100ml dosimeter 10ml absorption pipette

UV spectrometer 1cm cell

high performance liquid chromatography

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