3.3蛋白质分析

第三章 第三节 蛋白质分析

从GenBank 数据库中下载一条植物钙调序列，用ANTHEPROT软件预测其特征序列，然后查阅相关资料，说明其特征序列的特性与功能。

LOCUS AC_000175 66004023 BP DNA LINEAR CON 06-AUG-2013

DEFINITION BOS TAURUS BREED HEREFORD CHROMOSOME 18, BOS_TAURUS_UMD_3.1, WHOLE

GENOME SHOTGUN SEQUENCE. ACCESSION AC_000175 GPC_000000187 VERSION AC_000175.1 GI:258513349 DBLINK BIOPROJECT: PRJNA33843 ASSEMBLY: GCF_000003055.4 KEYWORDS WGS; REFSEQ.

SOURCE BOS TAURUS (CATTLE) ORGANISM BOS TAURUS

EUKARYOTA; METAZOA; CHORDATA; CRANIATA; VERTEBRATA; EUTELEOSTOMI;

MAMMALIA; EUTHERIA; LAURASIATHERIA; CETARTIODACTYLA; RUMINANTIA;

PECORA; BOVIDAE; BOVINAE; BOS. REFERENCE 1 (BASES 1 TO 66004023)

AUTHORS ZIMIN,A.V., DELCHER,A.L., FLOREA,L., KELLEY,D.R., SCHATZ,M.C.,

PUIU,D., HANRAHAN,F., PERTEA,G., VAN TASSELL,C.P., SONSTEGARD,T.S.,

MARCAIS,G., ROBERTS,M., SUBRAMANIAN,P., YORKE,J.A. AND SALZBERG,S.L.

TITLE A WHOLE-GENOME ASSEMBLY OF THE DOMESTIC COW, BOS TAURUS JOURNAL GENOME BIOL. 10 (4), R42 (2009) PUBMED 19393038

COMMENT REFSEQ INFORMATION: THE REFERENCE SEQUENCE IS IDENTICAL TO GK000018.2.

ASSEMBLY NAME: BOS_TAURUS_UMD_3.1 (UMD3.1)

THE GENOMIC SEQUENCE FOR THIS REFSEQ RECORD IS FROM THE WHOLE GENOME REASSEMBLY RELEASED BY THE CENTER FOR BIOINFORMATICS AND

COMPUTATIONAL BIOLOGY, UNIVERSITY OF MARYLAND IN DECEMBER 2009. THE

ORIGINAL WHOLE GENOME SHOTGUN PROJECT HAS THE PROJECT ACCESSION

DAAA00000000.2. THE UMD3.1 ASSEMBLY WAS GENERATED USING GENOMIC

TRACES SUBMITTED BY THE BOVINE GENOME SEQUENCING PROJECT (PROJECT

ID 12555). IN THIS ASSEMBLY SYNTENY BETWEEN THE COW AND HUMAN GENOMES AND INDEPENDENT MAPPING DATA WERE USED TO ASSEMBLE ROUGHLY

99% OF THE GENOME ONTO THE 30 BOS TAURUS CHROMOSOMES.

##GENOME-ANNOTATION-DATA-START## ANNOTATION PROVIDER :: NCBI

ANNOTATION STATUS :: FULL ANNOTATION ANNOTATION VERSION :: BOS TAURUS ANNOTATION RELEASE 103

ANNOTATION PIPELINE :: NCBI EUKARYOTIC GENOME ANNOTATION

PIPELINE ANNOTATION SOFTWARE VERSION :: 5.1

ANNOTATION METHOD :: BEST-PLACED REFSEQ; GNOMON FEATURES ANNOTATED :: GENE; MRNA; CDS; NCRNA ##GENOME-ANNOTATION-DATA-END## FEATURES LOCATION/QUALIFIERS SOURCE 1..66004023

/ORGANISM=\ /MOL_TYPE=\ /DB_XREF=\ /CHROMOSOME=\ /BREED=\CONTIG

JOIN(NW_003104462.1:1..3210545,GAP(UNK100),NW_003080576.1:1..16487, GAP(UNK100),NW_003104463.1:1..8212699,GAP(UNK100),

NW_003080672.1:1..1678,GAP(UNK100),NW_003080673.1:1..2521, GAP(UNK100),NW_003080674.1:1..4816,GAP(UNK100),

NW_003104464.1:1..31414493,GAP(UNK100),NW_003081036.1:1..1922, GAP(UNK100),NW_003081037.1:1..4751,GAP(UNK100),

NW_003081038.1:1..3920,GAP(UNK100),NW_003104465.1:1..8147483, GAP(UNK100),NW_003081267.1:1..5300,GAP(UNK100),

NW_003104466.1:1..6608038,GAP(UNK100),NW_003081470.1:1..15428, GAP(UNK100),NW_003104467.1:1..74485,GAP(UNK100),

NW_003081475.1:1..2457,GAP(UNK100),NW_003081476.1:1..3502, GAP(UNK100),NW_003104468.1:1..252438,GAP(UNK100),

NW_003081480.1:1..6336,GAP(UNK100),NW_003081481.1:1..11985,

GAP(UNK100),NW_003104469.1:1..1844788,GAP(UNK100),

NW_003081527.1:1..6010,GAP(UNK100),NW_003081528.1:1..5016, GAP(UNK100),NW_003104470.1:1..226317,GAP(UNK100),

NW_003081538.1:1..13289,GAP(UNK100),NW_003104471.1:1..145192, GAP(UNK100),NW_003081543.1:1..1155,GAP(UNK100),

NW_003104472.1:1..278225,GAP(UNK100),NW_003081551.1:1..4023, GAP(UNK100),NW_003104473.1:1..215174,GAP(UNK100),

NW_003081556.1:1..62196,GAP(UNK100),NW_003104474.1:1..415273, GAP(UNK100),NW_003081568.1:1..12595,GAP(UNK100),

NW_003104475.1:1..79519,GAP(UNK100),NW_003081574.1:1..13966, GAP(UNK100),NW_003104476.1:1..243366,GAP(UNK100),

NW_003081585.1:1..4515,GAP(UNK100),NW_003081586.1:1..2600, GAP(UNK100),NW_003081587.1:1..49630,GAP(UNK100),

NW_003104477.1:1..17373,GAP(UNK100),NW_003081591.1:1..1060, GAP(UNK100),NW_003104478.1:1..17361,GAP(UNK100),

NW_003081596.1:1..4364,GAP(UNK100),NW_003081597.1:1..7719, GAP(UNK100),NW_003081598.1:1..6781,GAP(UNK100),

NW_003081599.1:1..5320,GAP(UNK100),NW_003081600.1:1..9869, GAP(UNK100),NW_003104479.1:1..59392,GAP(UNK100),

NW_003081603.1:1..1697,GAP(UNK100),NW_003081604.1:1..4544, GAP(UNK100),NW_003081605.1:1..6054,GAP(UNK100),

NW_003081606.1:1..43259,GAP(UNK100),NW_003081607.1:1..5067, GAP(UNK100),NW_003104480.1:1..1433083,GAP(UNK100),

NW_003081652.1:1..947,GAP(UNK100),NW_003104481.1:1..218641, GAP(UNK100),NW_003081674.1:1..990,GAP(UNK100), NW_003104482.1:1..2530669) //

自定义序列：

>PDB1QU3A LIGASE/RNA 06-JUL-99 (LENGTH= 880 RESIDUES)

DYEKTLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKNKGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYK

TMQGFYAPYVPGWDTHGLPIEQALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGDFNDPYITLKPEYEA

AQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFNVKDDKGVVDADAKFIIWTTTPWTIPSNV

AITVHPELKYGQYNVNGEKYIIAEALSDAVAEALDWDKASIKLEKEYTGKELEWVVAQHPFLDRESLVINGDHVTTDAGT

GCVHTAPGHGEDDYIVGQQYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITHSYPHDWRTK

KPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGEWVISRQRVWGVPLPVFYAENGEIIMTKETVN

HVADLFAEHGSNIWFEREAKDLLPEGFTHPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRG

WFNSSITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSSTDYLADVRISDEILKQT

SDDYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLLNRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYL

DYGKDILYIEQRDSHIRRSMQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKWRTF

MNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVSQVKVVDKLDDQATAYEHGDIVIEHA